Top 7 best biological laboratory science edition 2019
Finding the best biological laboratory science edition suitable for your needs isnt easy. With hundreds of choices can distract you. Knowing whats bad and whats good can be something of a minefield. In this article, weve done the hard work for you.
Finding the best biological laboratory science edition suitable for your needs isnt easy. With hundreds of choices can distract you. Knowing whats bad and whats good can be something of a minefield. In this article, weve done the hard work for you.
Best biological laboratory science edition
1. Laboratory Life: The Construction of Scientific Facts, 2nd Edition
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Princeton University PressDescription
This highly original work presents laboratory science in a deliberately skeptical way: as an anthropological approach to the culture of the scientist. Drawing on recent work in literary criticism, the authors study how the social world of the laboratory produces papers and other "texts,"' and how the scientific vision of reality becomes that set of statements considered, for the time being, too expensive to change. The book is based on field work done by Bruno Latour in Roger Guillemin's laboratory at the Salk Institute and provides an important link between the sociology of modern sciences and laboratory studies in the history of science.
2. Antibodies A Laboratory Manual, Second edition
Description
It has been 25 years since the now-classic Antibodies by Ed Harlow and David Lane appeared. While some things remain the same, many have changed. This second edition of Antibodies, edited by Edward A. Greenfield of the Dana-Farber Cancer Institute builds on the core strengths of the first edition, presenting clear and authoritative protocols with extensive background information and troubleshooting advice. The original introductory chapters have been recast and updated to take into account our current understanding of the immune system. The critical chapters on generating monoclonal antibodies and growing hybridomas, which demystified hybridoma generation, have been greatly expanded and updated to make these procedures easy to follow and adaptable to current research needs. The remaining chapters contain all new protocols and topics and reflect the progress in how antibodies are studied and used since the appearance of the original edition. These include:antibody purification and storage
engineering antibodies, including use of degenerate oligonucleotides, 5'-RACE, phage display, and mutagenesis
extensive labeling techniques
new immunoblotting protocols
the latest screening and labeling techniques
As Dr. Greenfield notes in his preface to this second edition:
"The Antibodies manual provided our laboratory with guidance in the form of protocols and recommendations for setting up a hybridoma facility. Everything we needed to know to make a monoclonal antibody was all there, neatly packaged in an easy-to-understand book...the second edition is intended to provide the necessary information and protocols to assist investigators with their first monoclonal antibody effort as well as to provide guidance for more experiences antibody makers who are having some difficulties with a particular project."
I have the first edition. Why should I buy the second edition?
While the core of the first edition appears in updated and recast form in the second edition, more than half of the second edition contains entirely new information and protocols to reflect the changes in the field since the first edition.
To whom is this edition addressed? Is this a book for immunologists?
As with the first edition, the second edition provides information and protocols for both the neophyte and the experienced investigator. It dissects the mysteries of producing the antibodies and reagents you need for your experiments with the needs of molecular biologists in mind. It is the perfect book for new members of your lab as it has sufficient background information to provide context as well as clear, step-by-step instructions for performing the needed experiments-both the why and the how for these techniques. And if things go wrong, there is extensive troubleshooting to diagnose the problem.
Are the protocols in this book better than those available for download on the Web?
Many of the protocols available on the Web are perfectly good, but there are many that are not. Reagents are expensive and deadlines are tight. CSHL Press manuals provide protocols that have been formulated and tested in the labs of leading investigators in the field. They are reliable and they work. And they include the context and troubleshooting information that many online protocols do not have.
3. Laboratory Manual for Human Biology: Concepts and Current Issues (8th Edition)
Description
The Laboratory Manual provides an emphasis on critical thinking and includes Safety Guidelines, Objectives, A List of Materials Needed, Topic Introduction, Activities with embedded questions, and Critical Thinking Questions.
4. Molecular Cloning: A Laboratory Manual (Fourth Edition): Three-volume set
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Used Book in Good ConditionDescription
Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential. Molecular Cloning, Fourth Edition, by the celebrated founding author Joe Sambrook and new co-author, the distinguished HHMI investigator Michael Green, preserves the highly praised detail and clarity of previous editions and includes specific chapters and protocols commissioned for the book from expert practitioners at Yale, U Mass, Rockefeller University, Texas Tech, Cold Spring Harbor Laboratory, Washington University, and other leading institutions. The theoretical and historical underpinnings of techniques are prominent features of the presentation throughout, information that does much to help trouble-shoot experimental problems. For the fourth edition of this classic work, the content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories. Core chapters from the third edition have been revised to feature current strategies and approaches to the preparation and cloning of nucleic acids, gene transfer, and expression analysis. They are augmented by 12 new chapters which show how DNA, RNA, and proteins should be prepared, evaluated, and manipulated, and how data generation and analysis can be handled. The new content includes methods for studying interactions between cellular components, such as microarrays, next-generation sequencing technologies, RNA interference, and epigenetic analysis using DNA methylation techniques and chromatin immunoprecipitation. To make sense of the wealth of data produced by these techniques, a bioinformatics chapter describes the use of analytical tools for comparing sequences of genes and proteins and identifying common expression patterns among sets of genes. Please take a look at our book preview site at molecularcloning.com. Building on thirty years of trust, reliability, and authority, the fourth edition of Molecular Cloning is the new gold standard--the one indispensable molecular biology laboratory manual and reference source.5. At the Bench: A Laboratory Navigator, Updated Edition
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Used Book in Good ConditionDescription
At the Bench is the unique and hugely successful handbook for living and working in the laboratory, an essential aid to understanding basic lab techniques and how research groups work at a human level. In this newly revised edition, chapters have been rewritten to accommodate the impact of computer technology and the Internet, not only on the acquisition and analysis of data, but also on its organization and presentation. Alternatives to the use of radiation have been expanded, and figures and illustrations have been redrawn to reflect changes in laboratory equipment and procedures.6. Epigenetics, Second Edition
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In many biological processes the regulation of gene expression involves epigenetic mechanisms. In this new edition of Epigenetics, 36 chapters written by experts in the field introduce and explain epigenetic effects from many perspectives. These include the varied molecular mechanisms underpinning epigenetic regulation, discussion of cellular processes that rely on this kind of regulation, and surveys of model organisms in which epigenetic effects have been most studied.The original chapters have all been rewritten and brand new chapters cover topics such as the structure, function, and dynamics of histone-modifying enzymes and histone-interacting proteins. Other chapters address chromatin remodeling, DNA methylation, siRNAs, and gene silencing; X-chromosome inactivation, dosage compensation, and imprinting; and epigenetics in microbes, plants, insects, and mammals.
How epigenetic mechanisms act in cell division and cell type specification, and how errors in these pathways contribute to cancer and other human diseases are also considered, along with the importance of epigenetics for induced pluripotency and reprogramming. In addition, new chapters describe the involvement of epigenetic processes in epigenetic inheritance, neuronal development, metabolism and signaling, responses to the environment, and long-range chromatin interactions. A series of short essays highlight important recent discoveries.
All the chapters provide conceptual illustrations that help readers understand epigenetic control. The book is thus a benchmark text for advanced undergraduate and graduate courses on gene regulation, as well as an essential resource for scientists interested in this rapidly moving field.
7. Guide for the Care and Use of Laboratory Animals: Eighth Edition (Laboratory Safety)
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National Academies PressDescription
A respected resource for decades, the Guide for the Care and Use of Laboratory Animals has been updated by a committee of experts, taking into consideration input from the scientific and laboratory animal communities and the public at large. The Guide incorporates new scientific information on common laboratory animals, including aquatic species, and includes extensive references. It is organized around major components of animal use:
- Key concepts of animal care and use. The Guide sets the framework for the humane care and use of laboratory animals.
- Animal care and use program. The Guide discusses the concept of a broad Program of Animal Care and Use, including roles and responsibilities of the Institutional Official, Attending Veterinarian and the Institutional Animal Care and Use Committee.
- Animal environment, husbandry, and management. A chapter on this topic is now divided into sections on terrestrial and aquatic animals and provides recommendations for housing and environment, husbandry, behavioral and population management, and more.
- Veterinary care. The Guide discusses veterinary care and the responsibilities of the Attending Veterinarian. It includes recommendations on animal procurement and transportation, preventive medicine (including animal biosecurity), and clinical care and management. The Guide addresses distress and pain recognition and relief, and issues surrounding euthanasia.
- Physical plant. The Guide identifies design issues, providing construction guidelines for functional areas; considerations such as drainage, vibration and noise control, and environmental monitoring; and specialized facilities for animal housing and research needs.
The Guide for the Care and Use of Laboratory Animals provides a framework for the judgments required in the management of animal facilities. This updated and expanded resource of proven value will be important to scientists and researchers, veterinarians, animal care personnel, facilities managers, institutional administrators, policy makers involved in research issues, and animal welfare advocates.